The Max Planck Institute of Biochemistry (MPIB) in Martinsried is one of the world's leading research institutions in the fields of biochemistry, cell and structural biology, and biomedical research. With approximately 30 scientific departments and research groups and about 750 employees, the MPIB is one of the largest institutes of the Max Planck Society.


The newly formed Lentiviral Core Facility is seeking a skilled and motivated




Application Scientist / Facility Head Lentiviral Core Facility (m/f/d)

Full-time
The Lentiviral Core Facility will provide lentivirus-based services to support the MPIB’s scientific communities' needs for gene editing and transfer, including large- and small-scale genetic screening.


You will play a key role in driving the innovative use of lentivirus platforms that cover a wide range of gene transfer and screening applications tailored to the needs of the investigators. You will also harmonize vector and screening strategies across the institute with the goals of efficiency matched with quality of results and, at the same time, optimize vectors and libraries. You will act as the customer’s primary contact for this service and will discuss project strategy with the end users. You will oversee the work of one technician and we expect a high level of initiative. Flexibility to adapt to an ever-changing user and project landscape is key to success in this role.


In this role, you will:



Modify and/or develop vectors for cDNA transfer and associated lentivirus particle production
Modify and/or develop vectors for single-target CRISPR-Cas9-based gene modification and provide advice to the investigators for quantifying targeting events
Develop and maintain a limited cell line collection for virus production and testing, including limited Cas9+ lines
Acquire and maintain CRISPR libraries for whole genome screening and focused libraries. The libraries will include loss-of-function CRISPR-Cas9 approaches and CRISPR-i/a libraries
Produce Lentivirus particles from defined and certified cell lines
In collaboration with the Next Generation Sequencing facility, develop protocols to quantify library guide representation and quality, prior to investigator-based screening and assist investigators with screening strategies to obtain the desired outcome(s)
Maintain detailed records of vectors and libraries in electronic form that are transferable within groups at the institute and provide a permanent record according to the gene technology guidelines and rules
Engage with investigators prior to project implementation, during and after the project is complete to ensure their needs are met and troubleshoot problems as needed
Adhere to biosafety requirements within the laboratory environment (i.e., S2) including documentation and record keeping of all activities including storage of plasmids, virus particles and waste disposal

A PhD in a relevant scientific field (e. g., molecular biology, biochemistry, chemistry or related disciplines)
Proven expertise and experience in molecular biology techniques. Experience with Lentivirus S2-based work is not required but would be considered an advantage
Flexibility and openness in project development, record keeping and accountability
Strong problem-solving abilities and a track record of troubleshooting complex experiments
Ability to acquire and optimize new approaches and incorporate new ways of thinking and experimentation
Ability to work in a small, focused team but interface with larger groups at MPIB as well as other core facilities at MPIB. Ideally, some experience in managing personnel.
Inclination to work with customers and their projects and to set up the administrative framework necessary for running an efficient service operation.
Fluency in English and German. German is necessary for record keeping and reporting in regard to biosafety regulations

An interesting and varied job at one of the largest institutes of the Max Planck Socie...